Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules.
Opt Express
; 20(7): 7338-49, 2012 Mar 26.
Article
em En
| MEDLINE
| ID: mdl-22453414
ABSTRACT
The ability to detect single molecules over the electronic noise requires high performance detector systems. Electron Multiplying Charge-Coupled Device (EMCCD) cameras have been employed successfully to image single molecules. Recently, scientific Complementary Metal Oxide Semiconductor (sCMOS) based cameras have been introduced with very low read noise at faster read out rates, smaller pixel sizes and a lower price compared to EMCCD cameras. In this study, we have compared the two technologies using two EMCCD and three sCMOS cameras to detect single Cy5 molecules. Our findings indicate that the sCMOS cameras perform similar to EMCCD cameras for detecting and localizing single Cy5 molecules.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Semicondutores
/
Carbocianinas
/
Técnicas de Sonda Molecular
/
Imagem Molecular
/
Microscopia de Fluorescência
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Opt Express
Assunto da revista:
OFTALMOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos