[Separation and morphological observation of mouse testis seminiferous tubule epithelium].

ABSTRACT

OBJECTIVE: To explore a simple and feasible technique to locate proteins during spermatogenesis. METHODS: Various germ cells and somatic cells were separated by collagenase I and DNase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and confocal microscopy. RESULTS: Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus. CONCLUSION: A simple method for locating proteins during spermatogenesis was successfully developed.