Mmi1 RNA surveillance machinery directs RNAi complex RITS to specific meiotic genes in fission yeast.
EMBO J
; 31(10): 2296-308, 2012 May 16.
Article
em En
| MEDLINE
| ID: mdl-22522705
ABSTRACT
RNA interference (RNAi) silences gene expression by acting both at the transcriptional and post-transcriptional levels in a broad range of eukaryotes. In the fission yeast Schizosaccharomyces pombe the RNA-Induced Transcriptional Silencing (RITS) RNAi complex mediates heterochromatin formation at non-coding and repetitive DNA. However, the targeting and role of RITS at other genomic regions, including protein-coding genes, remain unknown. Here we show that RITS localizes to specific meiotic genes and mRNAs. Remarkably, RITS is guided to these meiotic targets by the RNA-binding protein Mmi1 and its associated RNA surveillance machinery that together degrade selective meiotic mRNAs during vegetative growth. Upon sexual differentiation, RITS localization to the meiotic genes and mRNAs is lost. Large-scale identification of Mmi1 RNA targets reveals that RITS subunit Chp1 associates with the vast majority of them. In addition, loss of RNAi affects the effective repression of sexual differentiation mediated by the Mmi1 RNA surveillance machinery. These findings uncover a new mechanism for recruiting RNAi to specific meiotic genes and suggest that RNAi participates in the control of sexual differentiation in fission yeast.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
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Regulação Fúngica da Expressão Gênica
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Proteínas de Schizosaccharomyces pombe
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Complexo de Inativação Induzido por RNA
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Fatores de Poliadenilação e Clivagem de mRNA
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Genes Fúngicos
Tipo de estudo:
Prognostic_studies
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Screening_studies
Idioma:
En
Revista:
EMBO J
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
França