Optical nanoscopy: from acquisition to analysis.
Annu Rev Biomed Eng
; 14: 231-54, 2012.
Article
em En
| MEDLINE
| ID: mdl-22559319
ABSTRACT
Recent advances in far-field microscopy have demonstrated that fluorescence imaging is possible at resolutions well below the long-standing diffraction limit. By exploiting photophysical properties of fluorescent probe molecules, this new class of methods yields a resolving power that is fundamentally diffraction unlimited. Although these methods are becoming more widely used in biological imaging, they must be complemented by suitable data analysis approaches if their potential is to be fully realized. Here we review the basic principles of diffraction-unlimited microscopy and how these principles influence the selection of available algorithms for data analysis. Furthermore, we provide an overview of existing analysis strategies and discuss their application.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanotecnologia
/
Microscopia
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Annu Rev Biomed Eng
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos