Podophyllum hexandrum aqueous extract as a potential free radical scavenger.
Redox Rep
; 17(2): 54-62, 2012.
Article
em En
| MEDLINE
| ID: mdl-22564348
ABSTRACT
The present study was undertaken to evaluate the effect of the aqueous extract of Podophyllum hexandrum against free radical-mediated damage and also explore its anticancer activity. The extract exhibited significant activity in scavenging 1, 1-diphenyl-2-picryl-hydrazyl radicals, (â¢)OH radical-mediated DNA damage, and lipid peroxide production in rat liver microsomes. The extract was also tested for its reducing abilities. The activity of liver marker enzymes and antioxidant defense enzymes in rat liver homogenate was assessed in control and carbon tetrachloride (CCl(4))-treated animals. It was observed that CCl(4)-induced changes viz., increases in the activities of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase, a decrease in reduced glutathione as well as decreases in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase. All these parameters showed reversal when pretreated with aqueous extract of P. hexandrum. Podophylotoxin and etoposide are the two known anticancer agents derived from P. hexandrum and interestingly the aqueous extract of P. hexandrum showed a typical DNA ladder formation in HL-60 cells confirming its role as an inducer of apoptosis. The results obtained suggest that the plant extract exhibits inhibition of and free radical production and lipid peroxidation, increase in antioxidant enzyme activities, revealing its antioxidant properties, and is also able to show potent anticancer activity as depicted by its ability to cause fragmentation of DNA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Intoxicação por Tetracloreto de Carbono
/
Sequestradores de Radicais Livres
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Antineoplásicos Fitogênicos
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Redox Rep
Assunto da revista:
BIOQUIMICA
/
METABOLISMO
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Índia