Evaluating the thermostability of commercial fast real-time PCR master mixes.
Exp Mol Pathol
; 93(2): 261-3, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22579954
ABSTRACT
There is an increasing need for affordable, thermostable PCR reagents that can be used for diagnostic testing in resource limited settings. The development of point-of-care devices in such settings is highly dependent on the availability and efficacy of thermostable reagents. Here, we assess the thermostability of commercial, intercalating dye-based real-time PCR master mixes. We show that several of these master mixes have thermostability and robust performance at 20°C, 40°C, and 45°C for 6, 4 and 2 weeks, respectively. However, none of the master mixes that we evaluated was able to withstand more than 1 month at 45°C incubation. Our findings have implications for clinical diagnostics in the developing world where cold-chain delivery of diagnostic assays is difficult to guarantee.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Kit de Reagentes para Diagnóstico
/
DNA
/
Reação em Cadeia da Polimerase em Tempo Real
/
Temperatura Alta
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Exp Mol Pathol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos