G-rich sequence-specific recognition and scission of human genome by PNA/DNA hybrid G-quadruplex formation.

ABSTRACT

Hole in one A single peptide nucleic acid (PNA) effectively targets the G-rich region in double-stranded DNA through formation of a PNA/DNA hybrid G-quadruplex. Only one target site in the whole human genome was selectively cleaved by the hybrid G-quadruplex. Such site-selective scission of DNA is central to gene manipulation for molecular biology, biotechnology, and therapy.