µ-chain-deficient mice possess B-1 cells and produce IgG and IgE, but not IgA, following systemic sensitization and inhalational challenge in a fungal asthma model.
J Immunol
; 189(3): 1322-9, 2012 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-22732592
ABSTRACT
Allergic bronchopulmonary aspergillosis is often difficult to treat and results in morbidity associated with chronic airway changes. This study assessed the requirement for B cells and their products in the allergic pulmonary phenotype in a murine model of fungal allergic asthma that mimics allergic bronchopulmonary aspergillosis. C57BL/6 and µMT mice (assumed to lack peripheral B cells) were sensitized with Aspergillus fumigatus extract and challenged with two inhalation exposures of live conidia to induce airway disease. Airway hyperresponsiveness after methacholine challenge, peribronchovascular inflammation, goblet cell metaplasia, and fibrotic remodeling of the airways was similar between µMT mice and their wild-type counterparts (C57BL/6). Surprisingly, even in the absence of the µ-chain, these µMT mice produced IgE and IgG Abs, although the Abs induced did not have specificity for A. fumigatus Ags. In contrast, IgA was not detected in either the lavage fluid or serum of µMT mice that had been exposed to A. fumigatus. Our findings also reveal the existence of CD19(+)CD9(+)IgD(+) B-1 cells in the lungs of the µMT animals. These data show the µMT mice to have a developmental pathway independent of the canonical µ-chain route that allows for their survival upon antigenic challenge with A. fumigatus conidia, although this pathway does not seem to allow for the normal development of Ag-specific repertoires. Additionally, this study shows that IgA is not required for either clearance or containment of A. fumigatus in the murine lung, as fungal outgrowth was not observed in the µMT animals after multiple inhalation exposures to live conidia.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina A
/
Imunoglobulina E
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Imunoglobulina G
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Subpopulações de Linfócitos B
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Imunização
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Cadeias mu de Imunoglobulina
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Aspergilose Pulmonar
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Antígenos de Fungos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos