Identification of the Cdc48â¢20S proteasome as an ancient AAA+ proteolytic machine.
Science
; 337(6096): 843-6, 2012 Aug 17.
Article
em En
| MEDLINE
| ID: mdl-22837385
ABSTRACT
Proteasomes are the major energy-dependent proteolytic machines in the eukaryotic and archaeal domains of life. To execute protein degradation, the 20S core peptidase combines with the AAA+ ring of the 19S regulatory particle in eukarya or with the AAA+ proteasome-activating nucleotidase ring in some archaea. Here, we find that Cdc48 and 20S from the archaeon Thermoplasma acidophilum interact to form a functional proteasome. Cdc48 is an abundant and essential double-ring AAA+ molecular machine ubiquitously present in archaea, where its function has been uncertain, and in eukarya where Cdc48 participates by largely unknown mechanisms in diverse cellular processes, including multiple proteolytic pathways. Thus, proteolysis in collaboration with the 20S peptidase may represent an ancestral function of the Cdc48 family.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Thermoplasma
/
Adenosina Trifosfatases
/
Proteínas de Ciclo Celular
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Proteínas Arqueais
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Complexo de Endopeptidases do Proteassoma
/
Proteólise
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Science
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos