Osteopontin increases the expression of ß1, 4-galactosyltransferase-I and promotes adhesion in human RL95-2 cells.
Glycoconj J
; 29(5-6): 347-56, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22847114
ABSTRACT
Beta1, 4-Galactosyltransferase-I (ß1, 4-GalT-I), which transfers galactose from UDP-Gal to N-acetylglucosamine and N-acetylglucosamine-terminated oligosaccharides of N- and O-linked glycans in a ß(1-4) linkage, plays a critical role in cell adhesion, sperm-egg recognition, neurite growth, and tumor cell migration and invasion. Our previously experiments also show that ß1, 4-GalT-I was up-regulated by estrogens and some important cytokines of embryo implantation especially Interleukin-1 (IL-1), TGF-α and Leukemia Inhibitory Factor (LIF) in endometrial cells. In the receptive phase human uterus, osteopontin (OPN) is the most highly up-regulated extracellular matrix/adhesion molecule/cytokine. In this study, we demonstrated the correlated expression of OPN and ß1, 4-GalT-I in endometrium during early pregnancy, and recombinant human OPN (rhOPN) protein induced the ß1, 4-GalT-I up-regulation in RL95-2 cells. Inhibition of MEK/ERK, PI3K/AKT and NF-κB suppressed rhOPN-induced ß1, 4-GalT-I expression. In addition, rhOPN promoted the adhesion of blastocysts cells in vitro in ß1, 4-GalT-I-dependent manner. Moreover, the adhesion is greatly inhibited when ß1, 4-GalT-I was blocked with the specific antibody. Taken together, our data suggest that ß1, 4-GalT-I provides a mechanism to bridge embryo to endometrium during implantation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Implantação do Embrião
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Proteínas Recombinantes
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Regulação da Expressão Gênica no Desenvolvimento
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Endométrio
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Osteopontina
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Galactosiltransferases
Limite:
Animals
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Glycoconj J
Assunto da revista:
BIOQUIMICA
/
METABOLISMO
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China