Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch.
Virol J
; 9: 144, 2012 Aug 03.
Article
em En
| MEDLINE
| ID: mdl-22862784
ABSTRACT
Reverse transcription (RT)-PCR is now the standard method for typing group A rotaviruses (RVA) to monitor the circulating genotypes in a population. Selection of primers that can accurately type the circulating genotypes is crucial in the context of vaccine introduction and correctly interpreting the impact of vaccination on strain distribution. To our knowledge this study is the first report from Asia of misidentification of genotype G9 as G3 due to a primer-template mismatch. We tested two published G-genotype specific primers sets, designed by Gouvea and colleagues (Set A) and Iturriza-Gomara and colleagues (Set B) on RVA from Hong Kong and Sri Lanka. Among 52 rotaviruses typed as G3 by set A primers, 36 (69.2%) were identified as G9 by nucleotide sequencing and set B primers. Moreover, of 300 rotaviruses tested, 28.3% were untypable by set A primers whereas only 12.3% were untypable by set B primers. Our findings reinforce the need to periodically monitor the primers used for RVA genotyping.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por Rotavirus
/
Primers do DNA
/
Rotavirus
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Erros de Diagnóstico
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Child, preschool
/
Humans
/
Infant
/
Newborn
País/Região como assunto:
Asia
Idioma:
En
Revista:
Virol J
Assunto da revista:
VIROLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão