Separation and quantification of histamine and N tau-methylhistamine in brain extracts.
Biomed Chromatogr
; 4(6): 245-8, 1990 Nov.
Article
em En
| MEDLINE
| ID: mdl-2289049
ABSTRACT
Histamine and its N tau-methyl derivative can be separated from perchloric acid extracts of rat brain by high performance liquid chromatography on a C18 column under isocratic conditions eluting with 0.1 M sodium phosphate buffer containing 0.19 mM sodium dodecyl sulphate and 25% methanol. Using electrochemical detection, histamine and N tau-methylhistamine can be detected at levels of less than 40 pg/microL tissue extract (less than 1 pmol). The retention times for histamine and N tau-methylhistamine were 15 min and 23 min, respectively, at a flow rate of 1.2 mL/min, and both compounds eluted as acceptably sharp peaks. The concentrations of histamine and N tau-methylhistamine in brain from seven-day-old rats were found to be very similar to those obtained by other analytical procedures.
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Base de dados:
MEDLINE
Assunto principal:
Química Encefálica
/
Histamina
/
Metilistaminas
Limite:
Animals
Idioma:
En
Revista:
Biomed Chromatogr
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Espanha