Sensitive detection of H2O2 and H2O2-related reactant with Ru(bipy)(2)(7,8-dimethyl-dipyridophenazine)2+ and oligodeoxyribonucleotide.
Analyst
; 137(19): 4428-34, 2012 Oct 07.
Article
em En
| MEDLINE
| ID: mdl-22893889
ABSTRACT
A sensor for H(2)O(2) and H(2)O(2)-related reactant was constructed with oligonucleotides and Ru(bipy)(2)dppx(2+) (bipy = 2,2'-bipyridine, dppx = 7,8-dimethyl-dipyridophenazine), which was performed by converting the H(2)O(2)-induced DNA cleavage into the change of luminescence. The 'DNA light switch' Ru(bipy)(2)dppx(2+) could emit strong luminescence in the presence of dsDNA. DNA cleavage occurred upon addition of H(2)O(2) due to the Fenton reaction, which resulted in the decrease of the luminescence of Ru(bipy)(2)dppx(2+). Therefore, the luminescence intensity depended on the concentration of H(2)O(2) and H(2)O(2)-related reactants, and the detection limits for H(2)O(2), uric acid and cholesterol were 0.20 µM, 0.46 µM and 1.25 µM, respectively. The recovery varied between 94.0% and 105.0% when the assay was applied to the determination of uric acid and cholesterol in biological samples, which demonstrated the good practicability of the assay.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligodesoxirribonucleotídeos
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Fenazinas
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Rutênio
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Espectrometria de Fluorescência
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2,2'-Dipiridil
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Peróxido de Hidrogênio
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Analyst
Ano de publicação:
2012
Tipo de documento:
Article