Mode of action of the schistosomicide hycanthone: site of DNA alkylation.
Mol Biochem Parasitol
; 43(1): 89-95, 1990 Nov.
Article
em En
| MEDLINE
| ID: mdl-2290447
ABSTRACT
Condensation of hycanthone N-methylcarbamate (HNMC) with deoxyguanosine (dG) furnished a mixture of the N-1 and N2 adducts which were purified and characterized as their acetates. Condensation of HNMC with thymidine (T) gave the N-3 adduct in poor yield. Adenosine (A) and cytidine (C) did not react with HNMC. Incubation of schistosomes with either [3H]hycanthone (HC) or [3H]HNMC furnished DNA to which [3H]HC was covalently bound. The alkylated DNA was degraded enzymically and the radiolabeled nucleosides were separated using HPLC. Two major peaks were observed which coincided in retention time with the synthetic N-1 and N2 alkylated dG. Alkylated T was absent. Thus, the site of alkylation of DNA by either HC or HNMC is dG.
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Base de dados:
MEDLINE
Assunto principal:
Schistosoma mansoni
/
DNA
/
Hicantone
Limite:
Animals
Idioma:
En
Revista:
Mol Biochem Parasitol
Ano de publicação:
1990
Tipo de documento:
Article