Immunomagnetic purification of murine primordial germ cells.
Methods Mol Biol
; 925: 61-6, 2012.
Article
em En
| MEDLINE
| ID: mdl-22907490
ABSTRACT
Primordial germ cells (PGCs) play essential roles in both reproduction and development. In this chapter, we describe a method used in our laboratory for the immunopurification of PGCs from the mouse embryo. After dissection and disruption of the fetal gonad, PGCs are identified by a monoclonal antibody recognizing an epitope characteristic of pluripotent stem cells. After reaction with a paramagnetic bead-linked secondary antibody, the cell mixture is applied to a strong magnetic field. PGCs are recovered by release from the magnetic field. Purity is assessed by the alkaline phosphatase activity inherent to PGCs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Óvulo
/
Separação Celular
/
Imunoprecipitação
/
Células-Tronco Embrionárias
/
Campos Magnéticos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos