Adenovirus-mediated delivery of soluble ST2 attenuates ovalbumin-induced allergic asthma in mice.
Clin Exp Immunol
; 170(1): 1-9, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22943195
ABSTRACT
Allergic asthma is associated with excessive T helper type 2 (Th2) cells activation and airway hyperreactivity (AHR), implicated in the context of significant morbidity and mortality. Soluble ST2, a member of the interleukin (IL)-1 receptor family, has been shown to play a critical role in modulation of inflammatory disorders, yet the function of soluble ST2 in allergic inflammation remains unclear. In this study, we examined the possibility of regulating ovalbumin (OVA)-challenged airway inflammation by recombinant adenovirus-mediated sST2-Fc (Ad-sST2-Fc) gene transfer. Single intranasal administration of Ad-sST2-Fc before allergen challenge in OVA-immunized mice profoundly reduced serum immunoglobulin (Ig)E secretion, eosinophil infiltration and concentrations of IL-4, IL-5 and IL-13 in bronchoalveolar lavage fluid compared with administration of a control Ad vector. Histopathological examination of the lungs revealed that sST2-Fc over-expression markedly suppressed allergen-induced peribronchial inflammation and disruption of the alveolar architecture. Moreover, the beneficial effect of sST2-Fc in allergic lung inflammation is related to blocking the IL-33/ST2L signalling. Taken together, these results suggested that administration of Ad-sST2-Fc gene transfer may have therapeutic potential for the immunomodulatory treatment of OVA-mediated allergic pulmonary diseases.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Asma
/
Terapia Genética
/
Receptores de Interleucina
/
Vetores Genéticos
Idioma:
En
Revista:
Clin Exp Immunol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China