Genomic analysis of a key innovation in an experimental Escherichia coli population.
Nature
; 489(7417): 513-8, 2012 Sep 27.
Article
em En
| MEDLINE
| ID: mdl-22992527
ABSTRACT
Evolutionary novelties have been important in the history of life, but their origins are usually difficult to examine in detail. We previously described the evolution of a novel trait, aerobic citrate utilization (Cit(+)), in an experimental population of Escherichia coli. Here we analyse genome sequences to investigate the history and genetic basis of this trait. At least three distinct clades coexisted for more than 10,000 generations before its emergence. The Cit(+) trait originated in one clade by a tandem duplication that captured an aerobically expressed promoter for the expression of a previously silent citrate transporter. The clades varied in their propensity to evolve this novel trait, although genotypes able to do so existed in all three clades, implying that multiple potentiating mutations arose during the population's history. Our findings illustrate the importance of promoter capture and altered gene regulation in mediating the exaptation events that often underlie evolutionary innovations.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genoma Bacteriano
/
Evolução Molecular
/
Ácido Cítrico
/
Genômica
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Escherichia coli
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Nature
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos