Pseudomonas punonensis sp. nov., isolated from straw.
Int J Syst Evol Microbiol
; 63(Pt 5): 1834-1839, 2013 May.
Article
em En
| MEDLINE
| ID: mdl-23002045
ABSTRACT
During a study of the 'tunta' (frozen-dry potato) production process in Peru, a bacterial strain, LMT03(T), was isolated from the straw grass in which the potatoes are dried. This strain was classified into the genus Pseudomonas on the basis of the 16S rRNA gene sequence analysis, and is most closely related to Pseudomonas argentinensis CH01(T) with 99.3â% identity in this gene and 96â%, 92â% and 86â% identities in rpoB, rpoD and gyrB genes, respectively. Strain LMT03(T) has a single polar flagellum, like other related yellow-pigment-producing pseudomonads. The major quinone is Q-9. The major fatty acids are C18ââ1ω7c in summed feature 8 (40.82â%), C16ââ1ω6c/C16ââ1ω6c in summed feature 3 (23.72â%) and C16ââ0 (15.20â%). The strain produces oxidase but it does not produce gelatinase, indole, urease, arginine dihydrolase or ß-galactosidase. Catalase production was very weak after 28 and 48 h incubation on nutrient agar medium. Nitrate reduction is negative. It does not hydrolyse aesculin. The DNA G+C content is 57.8 mol%. DNA-DNA hybridization results showed lower than 52â% relatedness with respect to the type strain of P. argentinensis, CH01(T). These results, together with other phenotypic characteristics, support the definition of a novel species within the genus Pseudomonas, for which the name Pseudomonas punonensis sp. nov. is proposed. The type strain is LMT03(T) (â=âLMG 26839(T)â=âCECT 8089(T)).
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Pseudomonas
/
Poaceae
País/Região como assunto:
America do sul
/
Peru
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Peru