Characterization of physiological response and identification of associated genes under heat stress in rice seedlings.
Plant Physiol Biochem
; 61: 46-53, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-23037947
ABSTRACT
Global warming, which is caused by greenhouse gas emissions, makes food crops more vulnerable to heat stress. Understanding the heat stress-related mechanisms in crops and classifying heat stress-related genes can increase our knowledge in heat-resistant molecular biology and propel developments in molecular design breeding, which can help rice cope with unfavorable temperatures. In this study, we carried out a physiological analysis of rice plants after heat stress. The results show a dramatic increase in malondialdehyde contents and SOD activities. We successfully isolated 11 heat-related rice genes with known function annotation through DNSH, which is an improved SSH method for screening long cDNA fragments. The reanalysis of microarray data from public database revealed that all these genes displayed various expression patterns after heat stress, drought, cold and salt. Quantitative real-time reverse transcription PCR was also performed to validate the expression of these genes after heat stress. The expressions in 10 genes were all significantly changed except for contig 77, which is a CBL-interacting protein kinase. Several reports have been published about the members of the same gene family.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Estresse Fisiológico
/
Adaptação Fisiológica
/
Genes de Plantas
/
Estresse Oxidativo
/
Temperatura Alta
/
Proteínas de Choque Térmico
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Plant Physiol Biochem
Assunto da revista:
BIOQUIMICA
/
BOTANICA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China