The role of PKC/ERK1/2 signaling in the anti-inflammatory effect of tetracyclic triterpene euphol on TPA-induced skin inflammation in mice.
Eur J Pharmacol
; 698(1-3): 413-20, 2013 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-23099255
ABSTRACT
Inflammation underlies the development and progression of a number of skin disorders including psoriasis, atopic dermatitis and cancer. Therefore, novel antiinflammatory agents are of great clinical interest for prevention and treatment of these conditions. Herein, we demonstrated the underlying molecular mechanisms of the antiinflammatory activity of euphol, a tetracyclic triterpene isolated from the sap of Euphorbia tirucalli, in skin inflammation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in mice. Topical application of euphol (100 µg/ear) significantly inhibited TPA-induced ear edema and leukocyte influx through the reduction of keratinocyte-derived chemokine (CXCL1/KC) and macrophage inflammatory protein (MIP)-2 levels. At the intracellular level, euphol reduced TPA-induced extracellular signal-regulated protein kinase (ERK) activation and cyclooxygenase-2 (COX-2) upregulation. These effects were associated with euphol's ability to prevent TPA-induced protein kinase C (PKC) activation, namely PKCα and PKCδ isozymes. Our data indicate that topical application of euphol markedly inhibits the inflammatory response induced by TPA. Thus, euphol represents a promising agent for the management of skin diseases with an inflammatory component.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Dermatopatias
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Proteína Quinase C
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Acetato de Tetradecanoilforbol
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Sistema de Sinalização das MAP Quinases
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MAP Quinases Reguladas por Sinal Extracelular
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Lanosterol
Limite:
Animals
Idioma:
En
Revista:
Eur J Pharmacol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Brasil