Relaxant effects of the essential oil of Mentha pulegium L. in rat isolated trachea and urinary bladder.
J Pharm Pharmacol
; 64(12): 1777-84, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-23146041
ABSTRACT
OBJECTIVES:
We evaluated the relaxant activity of the essential oil of Mentha pulegium L. (EOMP) and pulegone in rat isolated tracheal and bladder smooth muscles.METHODS:
ISOMETRIC contractions of isolated tracheal and bladder strips from male Wistar rats were induced by KCl (K60; 60 mm) or acetylcholine (ACh; 10 µm). EOMP and its majory compound pulegone were incubated, after contracting agent, with the tissues in cumulating concentrations. KEYFINDINGS:
EOMP (3-300 µg/ml) inhibited the contractions induced by ACh and K60 in both tissues, but was more effective against the contractions induced by K60 in trachea (IC50 = 40.47 ± 3.27 µg/ml) compared with ACh. Its relaxant action rules out ganglia and NO participation. Pulegone (10(-7) to 10(-3 ) m) inhibited the contractions induced by ACh and K60 in both tissues. EOMP concentration-dependently inhibited the contractions evoked by addition of CaCl(2) in depolarised trachea, suggesting inhibition of extracellular calcium entry.CONCLUSIONS:
These findings suggests that EOMP induced relaxant responses in pre-contracted smooth muscles of rat trachea and bladder, which are likely to be mediated via inhibition of calcium entry, mainly by its major compound, pulegone. These effects are coherent with the popular use of EOMP as an antispasmodic agent.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Parassimpatolíticos
/
Traqueia
/
Bexiga Urinária
/
Óleos Voláteis
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Mentha pulegium
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Contração Muscular
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Músculo Liso
Limite:
Animals
Idioma:
En
Revista:
J Pharm Pharmacol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil