Identification of carbapenem-resistant Acinetobacter baumannii clones using infrared spectroscopy.
J Biophotonics
; 7(5): 287-94, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-23192934
ABSTRACT
In this work we assessed the discriminatory ability of Fourier-transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem-hydrolyzing class D ß -lactamases (CHDL)-producing Acinetobacter spp. (5 hospitals; 2001-2008) previously characterized by DNA-based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL-producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying blaOXA-58 , ST98 carrying blaOXA-24/40 and ST92 carrying blaOXA-23 . Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Carbapenêmicos
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Espectroscopia de Infravermelho com Transformada de Fourier
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Farmacorresistência Bacteriana
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Acinetobacter baumannii
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Antibacterianos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Biophotonics
Assunto da revista:
BIOFISICA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Portugal