Quantification of lactate in synovia by microchip with contactless conductivity detection.
Anal Biochem
; 434(1): 73-7, 2013 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-23201388
ABSTRACT
This article describes the determination of lactate in synovia by microchip capillary electrophoresis (MCE) integrated with contactless conductivity detection (CCD). The optimal running buffer consists of 10mM tris(hydroxymethyl)aminomethane, 1 mM HCl, and 0.1 mM hexadecyltrimethylammonium bromide (pH 9.1). The quantitative measurement of lactate in dilute synovia samples can be finished in less than 40s. The results indicated that the peak area had a good linear relationship with lactate concentration in the range of 20 to 1000 µM, and the correlation coefficient was 0.9984. The average recovery was from 96.6% to 106.1%, and the interday relative standard deviation was less than 4.0% (n=6). The limit of detection (signal/noise=3) reached 6.5 µM. To validate the assay results, we compared the current method with the high-performance liquid chromatography method by measuring lactate in synovia samples. The data analysis verified that there was no significant difference between the two methods. Due to significant features such as low cost, integration, and miniaturization, the MCE-CCD method may have great potential in clinical diagnosis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Líquido Sinovial
/
Ácido Láctico
/
Eletroforese em Microchip
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
/
Adult
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Humans
/
Middle aged
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2013
Tipo de documento:
Article