Circulating cell-free fetal DNA for the detection of RHD status and sex using reflex fetal identifiers.
Prenat Diagn
; 33(1): 95-101, 2013 Jan.
Article
em En
| MEDLINE
| ID: mdl-23225162
ABSTRACT
OBJECTIVE:
To determine the sensitivity and specificity of circulating cell-free fetal DNA in determining the fetal RHD status and fetal sex.METHODS:
Maternal blood was collected in each trimester of pregnancy from RhD negative nonalloimmunized women. Whole blood was centrifuged, separated into plasma and buffy coat, and frozen at -80°C. DNA analysis was conducted via allele-specific primer extensions for exons 4, 5, and 7 of the RHD gene and for a 37-base pair insertion in exon 4 (RHD pseudogene; psi) three Y-chromosome sequences (SRY, DBY, and TTY2), and an extraction control (TGIFL-like X/Y). RhD serotyping on cord blood and gender assessment of the newborns were entered into a Web-based database.RESULTS:
One hundred twenty women were enrolled. The median gestational age at the first venipuncture was 12.4 (range 10.6-13.9) weeks with 120 samples drawn; 118 samples were drawn at 17.6 (16-20.9) weeks; and 113 samples at 28.7 (27.9-33.9) weeks. Overall accuracy for RHD was 99.1%, 99.1%, and 98.1% for each trimester and was 99.1%, 99.1%, and 100% for fetal sex determination.CONCLUSIONS:
Fetal RHD genotyping and sex can be very accurately determined in all three trimesters using circulating cell-free fetal DNA in the maternal circulation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sistema do Grupo Sanguíneo Rh-Hr
/
Análise para Determinação do Sexo
/
DNA
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Tipagem e Reações Cruzadas Sanguíneas
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Sangue Fetal
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Female
/
Humans
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Male
/
Pregnancy
Idioma:
En
Revista:
Prenat Diagn
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos