In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection.
Fertil Steril
; 99(3): 897-902.e1, 2013 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-23246449
ABSTRACT
OBJECTIVE:
To study the chromosome constitution and in vitro development of embryos derived from monopronucleated (1PN) zygotes after intracytoplasmic sperm injection (ICSI).DESIGN:
Prospective study.SETTING:
Private assisted reproduction center. PATIENT(S) Fifty-four 1PN 2PB zygotes obtained from 48 ICSI cycles. INTERVENTION(S) Pronuclear diameter measured 18 ± 2 hours after ICSI and embryonic in vitro development assessed until the blastocyst stage or arrest; embryos disaggregated and all cells analyzed for chromosomes 13, 18, 21, X, and Y by fluorescent in situ hybridization (FISH). MAIN OUTCOME MEASURE(S) Pronuclear size, in vitro embryo development, and cytogenetic characteristics. RESULT(S) All the embryos were chromosomally abnormal (7.4% diploid mosaic, 16.7% haploid mosaics, 5.5% aneuploid mosaic, and 70.4% chaotic mosaic), and 35.2% showed a Y chromosome. No difference in the pronuclear size was observed according to the type of abnormality. Only 7.4% of the embryos resulted in blastocysts with good morphologic features. CONCLUSION(S) Despite showing only one pronucleus, most of the zygotes resulted from fertilization. Embryos derived from 1PN ICSI zygotes are chromosomally abnormal. Neither the pronuclear size nor the in vitro developmental ability of these embryos can be predictive of their chromosomal constitution. According to our results, embryos that develop from 1PN ICSI zygotes should be discarded for any reproductive purpose.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Zigoto
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Aberrações Cromossômicas
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Diagnóstico Pré-Implantação
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Injeções de Esperma Intracitoplásmicas
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Desenvolvimento Embrionário
Tipo de estudo:
Diagnostic_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Fertil Steril
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Espanha