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Assembly and function of the regulator of G protein signaling 14 (RGS14)·H-Ras signaling complex in live cells are regulated by Gαi1 and Gαi-linked G protein-coupled receptors.
Vellano, Christopher P; Brown, Nicole E; Blumer, Joe B; Hepler, John R.
Afiliação
  • Vellano CP; Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
J Biol Chem ; 288(5): 3620-31, 2013 Feb 01.
Article em En | MEDLINE | ID: mdl-23250758
Regulator of G protein signaling 14 (RGS14) is a multifunctional scaffolding protein that integrates heterotrimeric G protein and H-Ras signaling pathways. RGS14 possesses an RGS domain that binds active Gα(i/o)-GTP subunits to promote GTP hydrolysis and a G protein regulatory (GPR) motif that selectively binds inactive Gα(i1/3)-GDP subunits to form a stable heterodimer at cellular membranes. RGS14 also contains two tandem Ras/Rap binding domains (RBDs) that bind H-Ras. Here we show that RGS14 preferentially binds activated H-Ras-GTP in live cells to enhance H-Ras cellular actions and that this interaction is regulated by inactive Gα(i1)-GDP and G protein-coupled receptors (GPCRs). Using bioluminescence resonance energy transfer (BRET) in live cells, we show that RGS14-Luciferase and active H-Ras(G/V)-Venus exhibit a robust BRET signal at the plasma membrane that is markedly enhanced in the presence of inactive Gα(i1)-GDP but not active Gα(i1)-GTP. Active H-Ras(G/V) interacts with a native RGS14·Gα(i1) complex in brain lysates, and co-expression of RGS14 and Gα(i1) in PC12 cells greatly enhances H-Ras(G/V) stimulatory effects on neurite outgrowth. Stimulation of the Gα(i)-linked α(2A)-adrenergic receptor induces a conformational change in the Gα(i1)·RGS14·H-Ras(G/V) complex that may allow subsequent regulation of the complex by other binding partners. Together, these findings indicate that inactive Gα(i1)-GDP enhances the affinity of RGS14 for H-Ras-GTP in live cells, resulting in a ternary signaling complex that is further regulated by GPCRs.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Proto-Oncogênicas p21(ras) / Receptores Adrenérgicos beta 2 / Receptores Adrenérgicos alfa 2 / Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP / Proteínas RGS Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Biol Chem Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Proto-Oncogênicas p21(ras) / Receptores Adrenérgicos beta 2 / Receptores Adrenérgicos alfa 2 / Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP / Proteínas RGS Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Biol Chem Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Estados Unidos