Surveillance of Japanese encephalitis virus infection in mosquitoes in Vietnam from 2006 to 2008.
Am J Trop Med Hyg
; 88(4): 681-8, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23358634
ABSTRACT
Japanese encephalitis virus (JEV) infection in mosquitoes was monitored in Vietnam from 2006 to 2008. A total of 15,225 mosquitoes, identified as 26 species in five genera were collected and 12,621 were grouped into 447 pools for examination of JEV infection by assays for cytopathic effects in C6/36 cells and by RT-PCR to detect flavivirus RNA. Three JEV strains were isolated from Culex tritaeniorhynchus Giles collected in northern and southern Vietnam and two JEV strains were isolated from Culex vishnui Theobald collected in the highlands of Vietnam. Genetic and phylogenetic analyses, based on complete E gene nucleotide sequences, revealed that the five JEV strains were classified into the genotype I group and six amino acid differences were found in these five strains. These results indicated that multiple JEV genotype I populations are circulating countrywide in Vietnam, transmitted by bites of their Cx. tritaeniorhynchus and Cx. vishnui.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Culex
/
Vírus da Encefalite Japonesa (Espécie)
/
Insetos Vetores
Tipo de estudo:
Screening_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Am J Trop Med Hyg
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão