Fluorescence-activated cell sorting-based quantitation of T cell receptor restimulation-induced cell death in activated, primary human T cells.
Methods Mol Biol
; 979: 15-23, 2013.
Article
em En
| MEDLINE
| ID: mdl-23397384
ABSTRACT
After initial stimulation with antigen and exposure to the growth cytokine interleukin-2, activated T lymphocytes become sensitized to apoptosis upon antigen restimulation through the T cell receptor. This self-regulatory, restimulation-induced cell death (RICD) program constrains the proliferative capacity of activated T cells to help prevent excessive T cell accumulation and associated immunopathology. Here we describe a simple FACS-based approach for measuring RICD sensitivity in activated human T cells following polyclonal restimulation in vitro. This procedure is a straightforward research and clinical diagnostic tool for assessing RICD sensitivity for T cells derived from normal donors and patients suffering from diseases causing dysregulated T cell homeostasis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores de Antígenos de Linfócitos T
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Linfócitos T
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Apoptose
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Citometria de Fluxo
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos