A ß-mercaptoethanol-modified enzyme-linked immunosorbent assay for diagnosis of canine visceral leishmaniasis.
J Vet Diagn Invest
; 25(2): 239-42, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23417077
ABSTRACT
Two immunoglobulin G enzyme-linked immunosorbent assay (ELISA) versions using whole promastigotes of Leishmania infantum (syn. Leishmania chagasi) treated either with ß-mercaptoethanol (ß-ME-ELISA) or trypsin (TRYP-ELISA) as antigens were developed for the diagnosis of canine visceral leishmaniasis (CVL). By comparison with the direct agglutination test (DAT; 100%, 31/31; 95% confidence interval [CI] 86.3-100%), slightly lower sensitivity was demonstrated for the newly developed ß-ME-ELISA (93.5%, 29/31; 95% CI 77.2-98.9%). Sensitivity was higher for ß-ME-ELISA compared with TRYP-ELISA (87.1%, 27/31; 95% CI 69.2-95.8%) in serum samples from dogs with CVL. When tested with sera from 37 healthy dogs and from 45 dogs with clinical conditions other than CVL, a specificity of 97.6% (80/82; 95% CI 90.1-99.6%) was estimated for ß-ME-ELISA as compared to 100% (82/82; 95% CI 94.4-100%) and 95.1% (78/82; 95% CI 87.3-98.4%) for DAT and TRYP-ELISA, respectively. Observed agreement was 94.0% (95% CI 88.7-97.1%) between DAT and ß-ME-ELISA (κ = 0.879; 95% CI 0.803-0.956) and 87.4% (95% CI 80.8-92.1%) between DAT and TRYP-ELISA (κ = 0.743; 95% CI 0.636-0.851). Current results advocate application of the new ß-ME-ELISA for diagnosis of CVL at the laboratory level and confirmation of results obtained with the DAT in field studies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Doenças do Cão
/
Leishmaniose Visceral
/
Mercaptoetanol
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Vet Diagn Invest
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Holanda