A fully attenuated recombinant Salmonid alphavirus becomes pathogenic through a single amino acid change in the E2 glycoprotein.
J Virol
; 87(10): 6027-30, 2013 May.
Article
em En
| MEDLINE
| ID: mdl-23449806
ABSTRACT
A recombinant sleeping disease virus (rSDV) was previously shown to be totally attenuated and provide long-term protection in trout (C. Moriette, M. Leberre, A. Lamoureux, T. L. Lai, M. Brémont, J. Virol. 804088-4098, 2006). Sequence comparison of the rSDV to wild-type genomes exhibited a number of nucleotide changes. In the current study, we demonstrate that the virulent phenotype of SDV was essentially associated with two amino acid changes, V8A and M136T, in the E2 glycoprotein, with the V8A change mostly being involved in the acquisition of the virulent phenotype.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Glicoproteínas
/
Alphavirus
/
Substituição de Aminoácidos
/
Mutação de Sentido Incorreto
/
Fatores de Virulência
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Virol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
França