Reversible protein affinity-labelling using bromomaleimide-based reagents.
Org Biomol Chem
; 11(15): 2408-11, 2013 Apr 21.
Article
em En
| MEDLINE
| ID: mdl-23462873
ABSTRACT
Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild conditions of using a stoichiometric amount of a bis-thiol, thus providing an approach that will be of general interest in chemical biology and proteomics.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biotina
/
Marcadores de Afinidade
/
Estreptavidina
/
Maleimidas
Idioma:
En
Revista:
Org Biomol Chem
Assunto da revista:
BIOQUIMICA
/
QUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Reino Unido