Cellular basis of burn-induced cardiac dysfunction and prevention by mesenteric lymph duct ligation.
J Surg Res
; 183(2): 678-85, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23465433
ABSTRACT
BACKGROUND:
Myocardial contractile depression develops 4 to 24 h after major burn injury. We have reported previously that in a rat burn injury model (≈40% of total body surface area burn), mesenteric lymph duct ligation (LDL) prior to burn prevented myocardial dysfunction. However, the underlying cellular and molecular mechanisms are not well understood. MATERIALS ANDMETHODS:
Left ventricular myocytes were isolated from sham burn (control), sham burn with LDL (sham + LDL), burn, and burn with LDL (burn + LDL) rats at 4 and 24 h after burn or sham burn. Electrophysiological techniques were used to study myocyte size, contractility and L-type Ca2+ channel current (ICa). Further studies examined changes in the messenger RNA expression levels of pore-forming subunit of the L-type Ca(2+) channel, α1C, and its auxiliary subunits, ß1, ß2, ß3, and α2δ1, which modulate the abundance of the ICa in post-burn hearts.RESULTS:
Depressed myocyte contractility (≈20%) developed during 4 to 24 h post-burn compared with control, sham + LDL, or burn + LDL groups, a pattern of changes consistent with whole heart studies. There was no significant alteration in myocyte size. The ICa density was significantly decreased (≈30%) at 24 h post-burn, whereas the messenger RNA expression levels of Ca(2+) channel gene were not significantly altered at 4 and 24 h after burn injury.CONCLUSIONS:
These results suggest that the post-burn contractile phenotype in vivo was also present in isolated myocytes in vitro, but cellular remodeling was not a major factor. The results also suggest that changes in ICa regulation, but not from Ca(2+) channel gene modification, may be a key element involved in post-burn contractile depression and the beneficial effects of LDL.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Queimaduras
/
Miócitos Cardíacos
/
Coração
/
Sistema Linfático
/
Mesentério
/
Contração Miocárdica
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Surg Res
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos