Bacterial translocation in an experimental model of multiple organ dysfunctions.
J Surg Res
; 183(2): 686-94, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23481560
ABSTRACT
BACKGROUND:
In order to investigate the hypothesis that bacterial translocation from the intestine contributes to death after multiple organ dysfunction syndrome (MODS), a sterile MODS model was studied.METHODS:
MODS was induced in 139 male C57BL/6 mice by lipopolysaccharide (LPS) (endotoxin) infusion followed by zymozan infusion in four groups Α, sham-operation; Β, LPS; C, LPS + 0.8 g/kg zymozan; and D, LPS + 1.2 g/kg zymozan. Mice were sacrificed at 24 and 48 h for quantitative tissue cultures, isolation, and stimulation of splenocytes, measurement of apoptosis of lymphocytes and macrophages, and of serum LPS and survival. Some mice with MODS were treated with the antibiotic ertapenem.RESULTS:
Enterobacteriaceae and Enterococcus spp were isolated from tissues. Group D had the highest bacterial load and the shortest survival. Release of interleukin-10, of interleukin-17, and of intgerferon-γ by splenocytes and the rate of apoptosis did not concur with immune paralysis. Serum LPS concentrations were higher in mice with MODS versus controls. Ertapenem prolonged survival and decreased the bacterial load.CONCLUSIONS:
Bacterial translocation seems to be an important contributor leading from MODS to death and suggests a change in therapy towards adaptation of antimicrobial treatment upon early signs of MODS.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Enterococcus
/
Translocação Bacteriana
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Modelos Animais de Doenças
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Enterobacteriaceae
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Insuficiência de Múltiplos Órgãos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Surg Res
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Grécia