Monitoring and quantifying dynamic physiological processes in live neurons using fluorescence recovery after photobleaching.
J Neurochem
; 126(2): 213-22, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-23496032
ABSTRACT
The direct visualization of subcellular dynamic processes is often hampered by limitations in the resolving power achievable with conventional microscopy techniques. Fluorescence recovery after photobleaching has emerged as a highly informative approach to address this challenge, permitting the quantitative measurement of the movement of small organelles and proteins in living functioning cells, and offering detailed insights into fundamental cellular phenomena of physiological importance. In recent years, its implementation has benefited from the increasing availability of confocal microscopy systems and of powerful labeling techniques based on genetically encoded fluorescent proteins or other chemical markers. In this review, we present fluorescence recovery after photobleaching and related techniques in the context of contemporary neurobiological research and discuss quantitative and semi-quantitative approaches to their interpretation.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
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Recuperação de Fluorescência Após Fotodegradação
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Fotodegradação
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Fenômenos Fisiológicos
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Neurônios
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Neurochem
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Reino Unido