Influence of beta-adrenergic stimulation on glycosylation of a major, secretory N-linked glycoprotein from rat parotid salivary gland.
Arch Oral Biol
; 35(3): 201-7, 1990.
Article
em En
| MEDLINE
| ID: mdl-2350264
ABSTRACT
beta-Adrenergic stimulation with 10 microM isoproterenol increased the [3H]-mannose/[14C]-leucine ratio (three- to six-fold) of protein extracts in double-radiolabelled rat parotid acinar cells. Characteristics of oligosaccharides in a major parotid glycoprotein (Mr approximately 220,000; gp 220) were studied. Gp 220 from control and experimental cells was endoglycosidase H-insensitive, endoglycosidase F-sensitive and bound both concanavalin A and wheat germ agglutinin. Gp 220 was removed from concanavalin A-Sepharose by sequential elution with 10 mM alpha-methyl glucoside and 0.5 M alpha-methyl mannoside. These findings suggest that (1) oligosaccharides in gp 220 have both a biantennary complex and hybrid oligosaccharide chains, and (2) beta-adrenoreceptor stimulation has little effect on the gross oligosaccharide structures of this glycoprotein.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Glândula Parótida
/
Proteínas e Peptídeos Salivares
/
Glicoproteínas
/
Isoproterenol
Limite:
Animals
Idioma:
En
Revista:
Arch Oral Biol
Ano de publicação:
1990
Tipo de documento:
Article