Determination of burn patient outcome by large-scale quantitative discovery proteomics.
Crit Care Med
; 41(6): 1421-34, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23507713
ABSTRACT
OBJECTIVES:
Emerging proteomics techniques can be used to establish proteomic outcome signatures and to identify candidate biomarkers for survival following traumatic injury. We applied high-resolution liquid chromatography-mass spectrometry and multiplex cytokine analysis to profile the plasma proteome of survivors and nonsurvivors of massive burn injury to determine the proteomic survival signature following a major burn injury.DESIGN:
Proteomic discovery study.SETTING:
Five burn hospitals across the United States. PATIENTS Thirty-two burn patients (16 nonsurvivors and 16 survivors), 19-89 years old, were admitted within 96 hours of injury to the participating hospitals with burns covering more than 20% of the total body surface area and required at least one surgical intervention.INTERVENTIONS:
None. MEASUREMENTS AND MAINRESULTS:
We found differences in circulating levels of 43 proteins involved in the acute-phase response, hepatic signaling, the complement cascade, inflammation, and insulin resistance. Thirty-two of the proteins identified were not previously known to play a role in the response to burn. Interleukin-4, interleukin-8, granulocyte macrophage colony-stimulating factor, monocyte chemotactic protein-1, and ß2-microglobulin correlated well with survival and may serve as clinical biomarkers.CONCLUSIONS:
These results demonstrate the utility of these techniques for establishing proteomic survival signatures and for use as a discovery tool to identify candidate biomarkers for survival. This is the first clinical application of a high-throughput, large-scale liquid chromatography-mass spectrometry-based quantitative plasma proteomic approach for biomarker discovery for the prediction of patient outcome following burn, trauma, or critical illness.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Queimaduras
/
Proteínas Sanguíneas
/
Citocinas
/
Mediadores da Inflamação
/
Proteômica
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Adult
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Aged
/
Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Crit Care Med
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos