Activation of different split functionalities on re-association of RNA-DNA hybrids.
Nat Nanotechnol
; 8(4): 296-304, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23542902
ABSTRACT
Split-protein systems, an approach that relies on fragmentation of proteins with their further conditional re-association to form functional complexes, are increasingly used for various biomedical applications. This approach offers tight control of protein functions and improved detection sensitivity. Here we report a similar technique based on a pair of RNA-DNA hybrids that can be used generally for triggering different split functionalities. Individually, each hybrid is inactive but when two cognate hybrids re-associate, different functionalities are triggered inside mammalian cells. As a proof of concept, this work mainly focuses on the activation of RNA interference. However, the release of other functionalities (such as resonance energy transfer and RNA aptamer) is also shown. Furthermore, in vivo studies demonstrate a significant uptake of the hybrids by tumours together with specific gene silencing. This split-functionality approach presents a new route in the development of 'smart' nucleic acid-based nanoparticles and switches for various biomedical applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
RNA
/
Ácidos Nucleicos Heteroduplexes
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Nanotechnol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos