Your browser doesn't support javascript.
loading
Novel cis-acting element within the capsid-coding region enhances flavivirus viral-RNA replication by regulating genome cyclization.
Liu, Zhong-Yu; Li, Xiao-Feng; Jiang, Tao; Deng, Yong-Qiang; Zhao, Hui; Wang, Hong-Jiang; Ye, Qing; Zhu, Shun-Ya; Qiu, Yang; Zhou, Xi; Qin, E-De; Qin, Cheng-Feng.
Afiliação
  • Liu ZY; Department of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
J Virol ; 87(12): 6804-18, 2013 Jun.
Article em En | MEDLINE | ID: mdl-23576500
cis-Acting elements in the viral genome RNA (vRNA) are essential for the translation, replication, and/or encapsidation of RNA viruses. In this study, a novel conserved cis-acting element was identified in the capsid-coding region of mosquito-borne flavivirus. The downstream of 5' cyclization sequence (5'CS) pseudoknot (DCS-PK) element has a three-stem pseudoknot structure, as demonstrated by structure prediction and biochemical analysis. Using dengue virus as a model, we show that DCS-PK enhances vRNA replication and that its function depends on its secondary structure and specific primary sequence. Mutagenesis revealed that the highly conserved stem 1 and loop 2, which are involved in potential loop-helix interactions, are crucial for DCS-PK function. A predicted loop 1-stem 3 base triple interaction is important for the structural stability and function of DCS-PK. Moreover, the function of DCS-PK depends on its position relative to the 5'CS, and the presence of DCS-PK facilitates the formation of 5'-3' RNA complexes. Taken together, our results reveal that the cis-acting element DCS-PK enhances vRNA replication by regulating genome cyclization, and DCS-PK might interplay with other cis-acting elements to form a functional vRNA cyclization domain, thus playing critical roles during the flavivirus life cycle and evolution.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Replicação Viral / RNA Viral / Regulação Viral da Expressão Gênica / Capsídeo / Elementos Facilitadores Genéticos / Genoma Viral / Flavivirus Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Virol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Replicação Viral / RNA Viral / Regulação Viral da Expressão Gênica / Capsídeo / Elementos Facilitadores Genéticos / Genoma Viral / Flavivirus Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Virol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: China