Emergence of whole-cell MALDI-MS biotyping for high-throughput bioanalysis of mammalian cells?
Bioanalysis
; 5(8): 885-93, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23590472
ABSTRACT
Since their inception in the 1970s, methods for classification of microorganisms based on mass spectral fingerprints obtained by MALDI-TOF MS have become a mainstay in environmental as well as in clinical microbiology. Recently, related whole-cell MALDI-TOF fingerprinting workflows have been adopted for the classification of mammalian cells. In this report we summarize this work and discuss the challenges of adapting whole-cell MS fingerprinting methods for the successful classification of mammalian cells. We highlight current limitations as well as opportunities and emerging applications of this technology in industrial and clinical settings, such as cell-line authentication, clinical diagnostics, and quality and productivity control in bioprocesses.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Alemanha