Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose.
Cancer Invest
; 31(5): 359-64, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23621632
ABSTRACT
Identification of measurable nontransient responses to low-dose radiation in human primary cell cultures remains a problem. To this end, circulating endothelial colony-forming (progenitor) cells (ECFCs) were examined as an experimental model. ECFCs were isolated from three cord blood donors. Cells were positive for endothelial cell markers and remained highly proliferative after long-term cryopreservation. A single dose of X-ray radiation (0.06-0.38 Gy) inhibited ECFC culture growth. This effect was evident at 48 hours and persisted up to 72 hr postirradiation. Such protracted cytostatic response of ECFCs to low-dose radiation suggests that ECFC primary cultures can be used to study low-dose radiation effects.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Células Endoteliais
/
Proliferação de Células
Limite:
Humans
Idioma:
En
Revista:
Cancer Invest
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos