Developmental toxicity and estrogenic potency of zearalenone in zebrafish (Danio rerio).
Aquat Toxicol
; 136-137: 13-21, 2013 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-23624394
ABSTRACT
Zearalenone (ZEA, F2) is one of the most common mycotoxins and the only known mycoestrogen. It enters the food and feed chain from contaminated cereals and infiltrates into sewage or natural waters posing potential threat to exposed livestock, wildlife and humans. Therefore evaluation of its biological effects is of international importance. We performed toxicological tests on zebrafish (Danio rerio) larvae and adults. Developmental toxicity was assessed by an extended (5 days) fish embryo toxicity test (FET). Effects of early ZEA exposure were concentration-dependent with LC50 and LC10 values of 893 and 335 µg/L. In larvae exposed to 500 µg/L and above, ZEA induced similar phenotype to has (heart-and soul) showing defects in heart and eye development and upward curvature of the body axis. From 250 µg/L at 72 hpf the gap in the melanophore streak at the base of the tail fin was missing and the fin fold was abnormal, suggesting disturbance in the development of the adult tail fin primordium. Estrogenic potency was measured on the basis of Vitellogenin (Vtg) protein (adults) levels and relative abundance of vitellogenin-1 mRNA (vtg-1) (larvae and adults). qRT-PCR in larvae proved to be sufficient substitute to adult tests and sensitive enough to detect ZEA in 0.1 µg/L concentrations, that is close to levels observed in wastewaters. Developmental defects reveal that besides direct estrogenic effects, zearalenone might interact with other ontogenic pathways.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fenótipo
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Zearalenona
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Peixe-Zebra
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Padronização Corporal
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Estrogênios não Esteroides
Limite:
Animals
Idioma:
En
Revista:
Aquat Toxicol
Assunto da revista:
BIOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Hungria