Chicken acrosin: extraction and purification.
Poult Sci
; 69(5): 812-7, 1990 May.
Article
em En
| MEDLINE
| ID: mdl-2367271
ABSTRACT
The objective of the present research was to identify a procedure whereby chicken acrosin could be purified. Acrosin, as evidenced by amidase activity, was extracted with urea most efficiently at a concentration of 6 M. Extraction efficiency was enhanced by spermatozoal lysis prior to admixture with 6 M urea. Lysis was induced by passage of spermatozoal suspensions through a French pressure cell. Acrosin was purified by using gel filtration, chromatofocusing, and affinity chromatography. Based on amidase activity, a 19-fold purification was obtained with a 28% recovery. Native electrophoresis resolved two major protein bands with proteolytic activity. The methods described afford the procurement of milligram amounts of chicken acrosin.
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Base de dados:
MEDLINE
Assunto principal:
Sêmen
/
Serina Endopeptidases
/
Acrosina
/
Galinhas
Limite:
Animals
Idioma:
En
Revista:
Poult Sci
Ano de publicação:
1990
Tipo de documento:
Article