An amplification-promoting sequence from mouse genomic DNA: interaction with a trans-acting factor that also affects gene expression.
DNA Cell Biol
; 9(5): 311-21, 1990 Jun.
Article
em En
| MEDLINE
| ID: mdl-2372375
ABSTRACT
We previously identified murine DNA sequences that stimulate the amplification of cis-linked plasmid DNA in mouse cells under selective conditions (Holst et al., 1988). Here we focus on the structural features of one of these elements, the 229-bp element 5. The amplification-promoting activity was fully recovered from the middle part of element 5. The active region interacted in a sequence-specific way with a protein from nuclear extracts. Using footprinting analyses the binding region was characterized and subsequently shown to be functionally active as an amplification-promoting sequence, whereas a mutated binding site was inactive. Therefore, cis-acting element 5 functioned via interaction with a trans-acting factor. The same binding site was also active as a promoter element for RNA polymerase II transcription, because it efficiently reconstituted the activity of a truncated herpes simplex virus type 1 (HSV-1) thymidine kinase (tk) gene promoter lacking the distal Sp1 binding site. Thus, the same protein seems to function in both RNA polymerase II transcription and DNA amplification. Possible relationships between both functions are discussed.
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Base de dados:
MEDLINE
Assunto principal:
Transativadores
/
Amplificação de Genes
/
Regulação da Expressão Gênica
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
DNA Cell Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Alemanha