[Peptides analysis in digested edible bird's nest by HPLC-MS].
Zhongguo Zhong Yao Za Zhi
; 38(5): 714-9, 2013 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-23724682
ABSTRACT
Edible bird's nest contains lots of glycoproteins. The glycosylation inhomogeneity for glycoprotein often results in wide range of molecular weight and the difficulty for protein separation and charaterization. In this paper, proteins in the edible bird's nest were extracted using multiple extractions, and then digested by PNgase F and trypsin. The digest mixture was separated with HPLC, and peptides were identified based on MS/MS data searching. The results indicated that the extracted proteins were amount to 79.7% of total protein in the edible bird's nest. More than 20 species of peptides in the digested mixture were identified. The sequences of these peptides showed similarity with some proteins from Swiss-prot. The research indicated that deglycosylation, tryptic digestion coupled with HPLC-MS/MS is a proper strategy for characterization of proteins in the edible bird's nest.
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Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Espectrometria de Massas
/
Aves
/
Proteólise
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China