Your browser doesn't support javascript.
loading
miRNA-548c: a specific signature in circulating PBMCs from dilated cardiomyopathy patients.
J Mol Cell Cardiol ; 62: 131-41, 2013 Sep.
Article em En | MEDLINE | ID: mdl-23735785
High fidelity genome-wide expression analysis has strengthened the idea that microRNA (miRNA) signatures in peripheral blood mononuclear cells (PBMCs) can be potentially used to predict the pathology when anatomical samples are inaccessible like the heart. PBMCs from 48 non-failing controls and 44 patients with relatively stable chronic heart failure (ejection fraction of ≤ 40%) associated with dilated cardiomyopathy (DCM) were used for miRNA analysis. Genome-wide miRNA-microarray on PBMCs from chronic heart failure patients identified miRNA signature uniquely characterized by the downregulation of miRNA-548 family members. We have also independently validated downregulation of miRNA-548 family members (miRNA-548c & 548i) using real time-PCR in a large cohort of independent patient samples. Independent in silico Ingenuity Pathway Analysis (IPA) of miRNA-548 targets shows unique enrichment of signaling molecules and pathways associated with cardiovascular disease and hypertrophy. Consistent with specificity of miRNA changes with pathology, PBMCs from breast cancer patients showed no alterations in miRNA-548c expression compared to healthy controls. These studies suggest that miRNA-548 family signature in PBMCs can therefore be used to detect early heart failure. Our studies show that cognate networking of predicted miRNA-548 targets in heart failure can be used as a powerful ancillary tool to predict the ongoing pathology.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Cardiomiopatia Dilatada / MicroRNAs Limite: Female / Humans / Male / Middle aged Idioma: En Revista: J Mol Cell Cardiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Cardiomiopatia Dilatada / MicroRNAs Limite: Female / Humans / Male / Middle aged Idioma: En Revista: J Mol Cell Cardiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Estados Unidos