Interfacial engineering of pyridinium gemini surfactants for the generation of synthetic transfection systems.
Biomaterials
; 34(28): 6906-21, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23768782
ABSTRACT
Pyridinium gemini surfactants possess a soft charge, a high charge/mass ratio and a high molecular flexibility - all key parameters that recommend their use in synthetic gene delivery systems with in vitro and in vivo efficiency. In present study we generated a DNA delivery system through interfacial engineering of pyridinium gemini surfactants at the level of linker, hydrophobic chains and counterions. The self-assembling of the pyridinium amphiphiles and the physicochemical properties of the resultant supra-molecular assemblies were studied in bulk and in solution through a combination of techniques that included DSC, X-ray diffraction, polarized microscopy, CMC, dynamic light scattering and zeta potential measurements. We assessed the impact of different structural elements and formulation parameters of these pyridinium amphiphiles on their DNA compaction properties, transfection efficiency, cytotoxicity, in a complete structure-activity relationship study. This interfacial engineering process generated transfection systems with reduced cytotoxicity and high transfection efficiency in media containing elevated levels of serum that mimic the in vivo conditions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos de Piridínio
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Tensoativos
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Transfecção
Limite:
Humans
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos