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Comparison of glioma stem cells to neural stem cells from the adult human brain identifies dysregulated Wnt- signaling and a fingerprint associated with clinical outcome.
Sandberg, Cecilie Jonsgar; Altschuler, Gabriel; Jeong, Jieun; Strømme, Kirsten Kierulf; Stangeland, Biljana; Murrell, Wayne; Grasmo-Wendler, Unn-Hilde; Myklebost, Ola; Helseth, Eirik; Vik-Mo, Einar Osland; Hide, Winston; Langmoen, Iver A.
Afiliação
  • Sandberg CJ; Vilhelm Magnus Laboratory, Institute for Surgical Research, University of Oslo, Norway. cesandbe@medisin.uio.no
Exp Cell Res ; 319(14): 2230-43, 2013 Aug 15.
Article em En | MEDLINE | ID: mdl-23791939
Glioblastoma is the most common brain tumor. Median survival in unselected patients is <10 months. The tumor harbors stem-like cells that self-renew and propagate upon serial transplantation in mice, although the clinical relevance of these cells has not been well documented. We have performed the first genome-wide analysis that directly relates the gene expression profile of nine enriched populations of glioblastoma stem cells (GSCs) to five identically isolated and cultivated populations of stem cells from the normal adult human brain. Although the two cell types share common stem- and lineage-related markers, GSCs show a more heterogeneous gene expression. We identified a number of pathways that are dysregulated in GSCs. A subset of these pathways has previously been identified in leukemic stem cells, suggesting that cancer stem cells of different origin may have common features. Genes upregulated in GSCs were also highly expressed in embryonic and induced pluripotent stem cells. We found that canonical Wnt-signaling plays an important role in GSCs, but not in adult human neural stem cells. As well we identified a 30-gene signature highly overexpressed in GSCs. The expression of these signature genes correlates with clinical outcome and demonstrates the clinical relevance of GSCs.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / Neoplasias Encefálicas / Regulação Neoplásica da Expressão Gênica / Glioblastoma / Células-Tronco Neurais / Via de Sinalização Wnt Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Humans / Middle aged Idioma: En Revista: Exp Cell Res Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Noruega

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / Neoplasias Encefálicas / Regulação Neoplásica da Expressão Gênica / Glioblastoma / Células-Tronco Neurais / Via de Sinalização Wnt Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Humans / Middle aged Idioma: En Revista: Exp Cell Res Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Noruega