High-fat and high-sucrose (western) diet induces steatohepatitis that is dependent on fructokinase.
Hepatology
; 58(5): 1632-43, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-23813872
ABSTRACT
UNLABELLED Fructose intake from added sugars has been implicated as a cause of nonalcoholic fatty liver disease. Here we tested the hypothesis that fructose may interact with a high-fat diet to induce fatty liver, and to determine if this was dependent on a key enzyme in fructose metabolism, fructokinase. Wild-type or fructokinase knockout mice were fed a low-fat (11%), high-fat (36%), or high-fat (36%) and high-sucrose (30%) diet for 15 weeks. Both wild-type and fructokinase knockout mice developed obesity with mild hepatic steatosis and no evidence of hepatic inflammation on a high-fat diet compared to a low-fat diet. In contrast, wild-type mice fed a high-fat and high-sucrose diet developed more severe hepatic steatosis with low-grade inflammation and fibrosis, as noted by increased CD68, tumor necrosis factor alpha, monocyte chemoattractant protein-1, alpha-smooth muscle actin, and collagen I and TIMP1 expression. These changes were prevented in the fructokinase knockout mice. CONCLUSION:
An additive effect of high-fat and high-sucrose diet on the development of hepatic steatosis exists. Further, the combination of sucrose with high-fat diet may induce steatohepatitis. The protection in fructokinase knockout mice suggests a key role for fructose (from sucrose) in this development of steatohepatitis. These studies emphasize the important role of fructose in the development of fatty liver and nonalcoholic steatohepatitis.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Sacarose
/
Fígado Gorduroso
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Dieta Hiperlipídica
/
Frutoquinases
Limite:
Animals
Idioma:
En
Revista:
Hepatology
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Colômbia