Transverse susceptibility as a biosensor for detection of Au-Fe3O4 nanoparticle-embedded human embryonic kidney cells.
Sensors (Basel)
; 13(7): 8490-500, 2013 Jul 03.
Article
em En
| MEDLINE
| ID: mdl-23823971
ABSTRACT
We demonstrate the possibility of using a radio-frequency transverse susceptibility (TS) technique based on a sensitive self-resonant tunnel-diode oscillator as a biosensor for detection of cancer cells that have taken up magnetic nanoparticles. This technique can detect changes in frequency on the order of 10 Hz in 10 MHz. Therefore, a small sample of cells that have taken up nanoparticles when placed inside the sample space of the TS probe can yield a signal characteristic of the magnetic nanoparticles. As a proof of the concept, Fe3O4 nanoparticles coated with Au (mean size ~60 nm) were synthesized using a micellar method and these nanoparticles were introduced to the medium at different concentrations of 0.05, 0.1, 0.5, and 1 mg/mL buffer, where they were taken up by human embryonic kidney (HEK) cells via phagocytosis. While the highest concentration of Au-Fe3O4 nanoparticles (1 mg/mL) was found to give the strongest TS signal, it is notable that the TS signal of the nanoparticles could still be detected at concentrations as low as 0.1 mg/mL.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Técnicas Biossensoriais
/
Separação Imunomagnética
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Nanopartículas de Magnetita
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Rastreamento de Células
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Sensors (Basel)
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos