Anti-androgen effects of the pyrethroid pesticide cypermethrin on interactions of androgen receptor with corepressors.
Toxicology
; 311(3): 178-83, 2013 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-23831764
ABSTRACT
To clarify whether the mechanism of androgen receptor (AR) antagonism of the pyrethroid pesticide cypermethrin associates with the interactions between the AR and corepressors silencing mediator for thyroid hormone receptors (SMRT) and nuclear receptor corepressor (NCoR), we have developed the mammalian two-hybrid assays. The AR N-terminal domain 1-660 amino acid residues were subcloned into the plasmid pVP16 to construct VP16-ARNTD. The C-terminal receptor interaction domains (RIDs) of SMRT and NCoR were used to construct pM-SMRT and pM-NCoR. The constructed vectors pVP16-ARNTD, pM-SMRT or pM-NCoR, the reporter pG5CAT and the control pCMVß were cotranfected into the CV-1 cells. The cells were treated with cypermethrin at the indicated concentrations. The AR N terminus interacted with RIDs of SMRT and NCoR. The interactions between the AR and corepressors SMRT and NCoR were enhanced by cypermethrin, and the significant enhancement was detected at the concentration of 10(-5)M. The mammalian two-hybrid assays demonstrate the utility to detect the interactions of the AR with SMRT and NCoR. Cypermethrin functions as an anti-androgen by enhancing the associations of the AR with SMRT and NCoR. We provide a novel mechanism in anti-androgen action of cypermethrin associated with the recruitment of SMRT and NCoR to AR.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Piretrinas
/
Receptores Androgênicos
/
Correpressor 1 de Receptor Nuclear
/
Correpressor 2 de Receptor Nuclear
/
Antagonistas de Androgênios
/
Inseticidas
Limite:
Animals
Idioma:
En
Revista:
Toxicology
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China